Intercept (PBS) Blocking Buffer and Antibody Diluent (927‑76003)

Intercept (TBS) Blocking Buffer and Antibody Diluent (927‑66003)

Specifications (Intercept Blocking Buffer)

• Intercept Blocking Buffer is a ready-to-use formulation. For optimal performance, do not dilute.
• Shelf Life: See expiration date on bottle.
• Storage: 4 °C
• Suitable for a variety of applications, including: quantitative Western blots, chemiluminescent Western blots, In‑Cell Western™ Assay, In-Gel Western, protein array, and glycoprotein detection.

Specifications (Intercept T20 Antibody Diluent)

• Shelf Life: See expiration date on bottle.

• Storage: 4 °C

• Contains a 0.05% concentration of Tween® 20.

  Because of the high purity and stability of the Tween 20 used in Intercept T20 Antibody Diluent, the concentration of 0.05% is sufficient. When using standard research grade Tween 20, use a concentration of 0.2%. See the NIR Western Blot Protocol (licorbio.com/wbanalysis) for more information.

• Suitable for a variety of applications, including: quantitative Western blots, chemiluminescent Western blots, In‑Cell Western™ Assay, In-Gel Western, protein array, and glycoprotein detection.

Product Description (Intercept Blocking Buffer)

Blocking buffers enhance the quality of Western blots by reducing background interference, increasing the signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions. Intercept Blocking Buffer is available in ready-to-use formulations in tris-buffered saline or phosphate-buffered saline to provide optimal blocking conditions for antibodies requiring TBS-based or PBS-based buffer systems.

The blocker does not contain mammalian proteins.

The TBS blocking buffer is ideal for use with antibodies against phosphorylated protein targets.

Description (Intercept T20 Antibody Diluent)

Intercept T20 Antibody Diluents improve specificity of primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Blocking Buffer or Intercept (TBS) Blocking Buffer preformulated with Tween® 20. When using a PVDF membrane, your secondary antibody diluent must contain a final concentration of 0.01 – 0.02% SDS.

Since there’s no need to mix the diluent yourself, you can save time and reduce steps in your protocol that might lead to variability in your results.

Related Products

See licorbio.com/intercept for ordering information.

Blocking Buffer

• Intercept (PBS) Protein-Free Blocking Buffer
• Intercept (TBS) Protein-Free Blocking Buffer

Antibody Diluent

• Intercept T20 (PBS) Protein-Free Antibody Diluent
• Intercept T20 (TBS) Protein-Free Antibody Diluent