IRDye® 680LT Goat Anti-Rabbit IgG

926-68021

Highly Cross-Adsorbed Goat (Polyclonal) Anti-Rabbit IgG (H+L) Antibody Conjugated to IRDye 680LT

Applications

IRDye 680LT secondary antibodies can be used for numerous applications, including Western blotting. IRDye 680LT secondary antibodies are also suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities. Two-color detection can be achieved by multiplexing IRDye 680LT secondary antibodies with IRDye 800CW secondary antibodies. These antibodies are optimized for use with Odyssey family imagers.

IRDye 680LT is not intended for in vivo applications.

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Storage

  • Storage temperature: 4 °C
  • Storage conditions: Protect from light and moisture.

  • Shelf life: When stored as recommended, this product is stable for 3 months.

Specifications

  • Fluorophore: IRDye 680LT (MW 1402)

  • Size:0.1 mg

  • Antibody Concentration:1.0 mg/mL(when reconstituted as directed)

  • Form of Antibody: IRDye 680LT purified immunoglobulin conjugate, lyophilized in phosphate buffered saline, pH 7.4. Contains 10 mg/mL BSA (free of IgG and protease) as stabilizer and 0.01% sodium azide as preservative, after reconstitution.

    Contains sodium azide.

  • Excitation Wavelength: 679 nm (in PBS)

  • Emission Wavelength: 696 nm (in PBS)

  • Immunogen: Rabbit IgG

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA. This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot.

Reconstitute Secondary Antibody

  1. Combine each 0.1 mg of secondary antibody with 0.1 mL of sterile distilled water.

  2. Gently mix by inverting.

  3. Allow the solution to stand at room temperature for at least 30 minutes before use. Protect the solution from light.

    If the solution is not completely dissolved after standing at room temperature, centrifuge the solution.

When stored as recommended, this product is stable for 3 months.

Recommended Dilutions

Optimum dilutions will vary and should be determined empirically.

Avoid non-specific background staining on PVDF membranes: If you are making your own antibody diluent, add SDS (final concentration 0.01 – 0.02%) and Tween® 20 (final concentration 0.2%) to the diluent. If using Intercept® T20 Antibody Diluent, only add SDS (final concentration 0.01 – 0.02%).

Recommended Dilutions for Western blotting

RecommendedSuggested Range
1:20,0001:10,000 – 1:50,000