IRDye® 800CW Streptavidin

Specifications

  • Size: 0.5 mg

  • Concentration: 1 mg/ml

  • Physical State: Liquid

  • Buffer: 10 mM Phosphate, 183 mM NaCl, and 2.7 mM KCl, pH 7.4

  • Preservative: Sodium azide 0.005% (w/v)

    Contains sodium azide.

  • IRDye 800CW Absmax = 774 nm, Emissionmax = 789 nm

  • IRDye 800CW Molecular Weight: 1166 g/mole

  • IRDye 800CW Extinction Coefficient = 240,000 M-1 cm-1 (in 1X PBS)

  • Storage: Store vial at 4 °C protected from light. Expiration date is six months from date received.

Protocol

IRDye 800CW Streptavidin can be used as a secondary detection reagent for microscopy, protein arrays, In-Gel Westerns, and Western blotting. Centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining.

Recommended Dilutions

A final concentration of 0.2 to 1.0 µg/mL (1:1,000 to 1:5,000) is usually satisfactory for most applications. However, appropriate dilution may need to be determined empirically. For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration) and Tween® 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.