COX IV Rabbit Monoclonal Antibody

Specifications (926-42214)

  • Size: 100 µL

  • Storage: -20 °C

  • Species Cross-Reactivity: human, rabbit, monkey, zebrafish, bovine, pig

    Not suitable for detection of COX IV derived from mouse.

  • Target Molecular Weight: 17 kDa

  • Specificity/Sensitivity: This antibody detects endogenous levels of total COX IV protein.

  • Immunogen: Animals are immunized with a synthetic peptide that corresponds to residues surrounding Lys29 of human COX IV.

  • Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide.

    Do not aliquot the antibody.

    Contains sodium azide.

Recommended Dilution

Western Blotting: 1:1000

Applications

Cytochrome c oxidase is localized to the inner mitochondrial membrane. The COX IV antibody can be used as a mitochondrial loading control and a loading control for normalizing low expressing target proteins. This COX IV primary antibody can be used for detection of COX IV by Western blot, or as a normalization antibody when performing two-color detection. Detection of this primary antibody can be achieved with IRDye® Goat Anti-Rabbit or IRDye Donkey Anti- Rabbit secondary antibodies.

See (licorbio.com/support) for detailed protocols.

Example Data

COX IV rabbit primary antibody example data
Figure 9. COX IV was detected in and COS-7, HeLa, and Jurkat cell lines. Dilutions of each cell lysate are: 2.5, 1.25, 0.625, 0.313, and 0.156 µg/well. Typical results are shown using Odyssey Nitrocellulose Membranes (P/N: 926-31092) and Intercept® Blocking Buffer (P/N: 927-70000). The COX IV antibody was used at a 1:1000 dilution and the IRDye 680RD Goat anti-Rabbit (P/N: 925-68071) was used at a 1:15,000 dilution (optimal range is 1:5000 – 1:25000). The Chameleon® Duo Pre-stained Protein Ladder (P/N: 928-60000) was used. The Western blot was imaged using the AutoScan function on an Odyssey Infrared Imaging System.