Histone H3 Rabbit Monoclonal Antibody

Specifications (926-42219)

  • Size: 100 µL

  • Storage: -20 °C

  • Species Cross-Reactivity: Human, mouse, rat, monkey

  • Target Molecular Weight: 17 kDa

  • Isotype: Rabbit IgG

  • Specificity/Sensitivity: Detects endogenous levels of total Histone H3 protein (including isoforms H3.1, H3.2, and H3.3) and Histone H3 variant CENP-A. Does not cross-react with other core histones. May cross-react with bovine, chicken, D. melanogaster, hamster, xenopus, and zebrafish.

  • Immunogen: A synthetic peptide that corresponds to the carboxy terminus of the human histone H3 protein

  • Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide

    Do not aliquot the antibody.

    Contains sodium azide.

  • Tested Application: Western blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Recommended Dilution

  • Western Blotting: 1:1000

Applications

The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.

The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.

Detect Histone H3 Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Histone H3 Rabbit Monoclonal Antibody in C32 Lysates

Histone H3 Rabbit Monoclonal Antibody detected in C32 cells
Figure 14. Histone H3 Rabbit Monoclonal Antibody was detected in C32 cells. C32 lysates were diluted from 2.5 µg to 156 ng. Lysates were separated on 4-12% Bis-Tris Gels electrophoresed at 200V for 45 minutes in MES Running Buffer and transferred to nitrocellulose membranes in Tris Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept (PBS) Blocking Buffer and probed with Histone H3 Rabbit Monoclonal Antibody and detected on an Odyssey DLx Imager.

Histone H3 Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and Cos7 Lysates

Histone H3 Rabbit Monoclonal Antibody detected in HeLa, NIH3T3, and COS7 lysates
Figure 15. Histone H3 Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS7 lysates. Lysates were diluted from 2.5 µg to 156 ng. Lysates were separated on 4-12% Bis-Tris Gels electrophoresed at 200V for 45 minutes in MES Running Buffer and transferred to nitrocellulose membranes in Tris Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept (PBS) Protein-Free Blocking Buffer and probed with Histone H3 Rabbit Monoclonal Antibody and detected on an Odyssey DLx Imager.