Vinculin Rabbit Monoclonal Antibody

Specifications (926-42215)

  • Size: 100 µL

  • Storage: -20 °C

  • Species Cross-Reactivity: Human, mouse, rat, monkey

  • Target Molecular Weight: 124 kDa

  • Isotype: Rabbit IgG

  • Specificity/Sensitivity: Detects endogenous levels of total vinculin protein and metavinculin, a 145 kDa splice variant of vinculin

  • Immunogen: A recombinant protein that is specific to the amino terminus of human vinculin protein

  • Storage Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide

    Do not aliquot the antibody.

    Contains sodium azide.

  • Tested Application: Western blot, Immunohistochemistry, Flow Cytometry

Recommended Dilution

  • Western Blotting: 1:1000

Applications

Vinculin is a highly conserved cytoskeletal protein. The Vinculin primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting high molecular weight targets.

The expression of Vinculin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.

Once validated, Vinculin primary antibodies can be used for the detection of Vinculin when performing multiplex Western blot detection.

Detect Vinculin Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.

Vinculin Rabbit Monoclonal Antibody Detected in C32 Cells

Vinculin Rabbit Monoclonal Antibody detected in C32 cells
Figure 16. Vinculin Rabbit Monoclonal Antibody detected in C32 cells. C32 lysates were diluted from 2.5 µg to 156 ng. Lysates were separated on 4-12% Bis-Tris Gels electrophoresed at 200V for 45 minutes in MES Running Buffer and transferred to nitrocellulose membranes in Tris Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept (PBS) Blocking Buffer and probed with Vinculin Rabbit Monoclonal Antibody and detected on an Odyssey DLx Imager.

Vinculin Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and Cos7 Lysates

Vinculin Rabbit Monoclonal Antibody detected in HeLa, NIH3T3, and COS7 lysates
Figure 17. Vinculin Rabbit Monoclonal Antibody detected in HeLa, NIH/3T3, and COS7 lysates. Lysates were diluted from 2.5 µg to 156 ng. Lysates were separated on 4-12% Bis-Tris Gels electrophoresed at 200V for 45 minutes in MES Running Buffer and transferred to nitrocellulose membranes in Tris Glycine buffer at 100V for 65 minutes. Blots were blocked in Intercept (PBS) Protein-Free Blocking Buffer and probed with Vinculin Rabbit Monoclonal Antibody and detected on an Odyssey DLx Imager.