Scan Custom

Topics in this section show the options for the Custom scanning workflow by imager model.

See the links below for emission/excitation data and example detection labels.

Odyssey M Imager Custom Scan

The following settings are available when using the Odyssey M Custom workflow.

  • Resolution Options: 5, 10, 20, 50 and 100 µm

    Scan area size can impact the available resolutions. For large scan areas, the resolution list on the Choose detection options page will include resolutions that will not produce a resultant image that exceeds the maximum size for the imaging system.

  • Focus Offset: -1.00 – 5.00 mm

  • Chemi membrane acquisition times: 30 sec, 1 min, 2 min (default), 5 min, 10 min, 20 min, 60 min

    Click the button to enter a custom acquisition time from 5 seconds to 60 minutes.

Choose detection options

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Channel Example Detection Labels Excitation Emission
800 IRDye 800CW, DyLight 800 785 nm 816 - 840 nm
700 IRDye 680LT, IRDye 680RD, IRDye 700, Revert 700 Total Protein Stain, Alexa Fluor 680, CellTag 700 Stain, DRAQ5 685 nm 721 - 740 nm
700A Custom 685 nm 816 – 840 nm
520 Revert 520 Total Protein Stain, CellTag 520 Stain, VRDye™ 549, Cy3, Alexa Fluor 546, Red fluorescent protein (RFP) 520 nm 570 - 610 nm
520A DRAQ5 520 nm 721 - 740 nm
520B Custom 520 nm 816 - 840 nm
488 VRDye 490, FITC, Green Fluorescent Protein (GFP) 488 nm 519 - 543 nm
488A SYPRO Ruby, SYPRO Orange 488 nm 570 - 610 nm
488B StarBright Blue 700 488 nm 721 - 740 nm
488C Custom 488 nm 816 - 840 nm
Chemi HRP and AP chemiluminescent substrates NA NA
RGB Epi Colorimetric Protein MW Markers, Ponceau S NA NA
630 Epi Pierce Reversible Protein Stain, TMB (blotting) 631 - 659 nm Clear Window
525 Epi Ponceau S, NBT/BCIP 500 - 530 nm Clear Window
470 Epi Custom 447 - 472 nm Clear Window
RGB Trans H&E Stain, Alcian Blue, Coomassie stain, Silver stain NA NA
630 Trans Coomassie stain, TMB ELISA (non- stopped) 631 - 659 nm Clear Window
525 Trans Custom 500 - 530 nm Clear Window
470 Trans Silver stain, TMB – stopped ELISA, OPD 447 - 472 nm Clear Window

Custom Scan for the Odyssey® F Imager

The following settings are available when using the Custom workflow.

  • Resolution Options: 21 μm, 42 μm, 84 μm, 169 μm, 337 μm

  • Focus Offset Range: -1.00 – 5.00 mm

Choose detection options

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Model Channel Excitation Emission
9150, 9180 800 785 nm 813 – 832 nm
9150, 9180 700 685 nm 710 – 730 nm
9150, 9180 700A 685 nm 813 – 832 nm
9180 520 520 nm 570 – 610 nm
9180 520A 520 nm 710 – 730 nm
9180 520B 520 nm 813 – 832 nm
9180 488 488 nm 538 – 552 nm
9180 488A 488 nm 570 – 610 nm
9180 488B 488 nm 710 – 730 nm
9180 488C 488 nm 813 – 832 nm

Custom Scan for the Odyssey DLx Imager or Odyssey CLx Imager

The following settings are available when using the Odyssey CLx Custom workflow.

  • Resolution Options: 21, 42, 84, 169, 337 µm

    Scan area size can impact the available resolutions. For large scan areas, the resolution list on the Choose detection options page will include resolutions that will not produce a resultant image that exceeds the maximum size for the imaging system.

  • Scan Quality Options: Lowest, Low, Medium, High, Highest

  • Intensity Options: Auto and Manual

  • Focus Offset Range: 0.00 – 4.00 mm

Choose detection options

The detection labels listed in this table are examples. If you do not see the detection label you want to use, check its spectral characteristics to make sure it is compatible.

Channel Labels Excitation Emission Combined Selections Allowed
800 IRDye® 800CW, DyLight 800 785 nm 812 – 832 nm 700
700 IRDye 680RD, IRDye 680LT, Alexa Fluor 680 685 nm 710 – 730 nm 800