CellTag 700 Stain Used for In-Cell Western Normalization

Have you ever wanted to try an in-cell ELISA but you just weren't sure how to get started?

With the new LI-COR^®CellTag™ 700 Stain, a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number, you have a easier — and more affordable — way to try this powerful application.

CellTag 700 Stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers (see Figure 1 below).

CellTag 700 Stain is applied to the cells during incubation with IRDye^® 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.

**Figure 1. Linear Relationship between Fluorescence and Cell Number.** Two-fold serial dilutions of A431 and NIH/3T3 cells were plated in 96-well plate, then fixed, permeabilized, stained with CellTag 700 Stain, and detected with Odyssey^® Classic (Resolution: 169um; Quality: medium; Focus offset: 4.0mm; Intensity: 5). The Trim Signals were used to generate the graphs.

CellTag 700 Stain ICW Kits offer a convenient way to try cell-based In-Cell Western™ Assays. Each kit includes blocking buffer, IRDye^® 800CW secondary antibody for detection of a specific protein target in the 800 nm channel, and CellTag 700 Stain to normalize well-to-well variations in cell number.

This cost-effective normalization method makes quantification of the target protein more precise.

**Figure 2. In-Cell Western Assay with CellTag 700 Stain in EGF-stimulated A431 Cells.** (Go to the CellTag 700 Stain Overview page for more details on this data). Try one of our new In-Cell Western Assay Kits with CellTag 700 Stain today and find out just how easy it is to perform fast, cost-effective cell-based Western assays.

Cell Stain Increases Ease of Use for In-Cell Western™ Normalization