NewBlot Western Blot Stripping Buffers are specially formulated for use with IRDye® infrared dyes (680RD, 680LT, and 800CW only) and the Odyssey® Infrared Imaging Systems to help you save time and money on recreating samples. NewBlot Stripping Buffer allows you to reuse the same blot by stripping and reprobing up to two fluorescent antibodies simultaneously.
So, you may ask, what is so great about NewBlot Stripping Buffer?
- Effectively removes antibodies, yet gentle enough to retain immobilized proteins
- Strips blots at room temperature in 20 minutes or less without an unpleasant odor
- Allows you to reuse the same blot up to 3X! (see the data below!)
- Offers qualitative analysis after stripping
NewBlot is available in three formulations: one for stripping nitrocellulose membranes, one for stripping PVDF membranes, and one that can be used for both PVDF and nitrocellulose membranes.
In the example below, beta tubulin and ERK2 were run on a gel and transferred to Immobilon®-FL PVDF membrane. They were probed with primary antibodies rabbit anti-beta-tubulin and mouse anti-ERK2 and then with IRDye 680 Goat anti-Rabbit (red) and IRDye 800CW Goat anti-Mouse (green), respectively.
NewBlot PVDF Stripping Buffer was used to strip the blot, which was then reprobed with the fluorescent secondary antibodies. This was repeated 2 more times. As you can see from the series of images, there is very little apparent loss of signal in either channel in the third blot as compared to the original blot.
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