In a perfect world, normalization would never be necessary, but no matter how hard we try, we cannot account for all variability.
Normalization makes the imperfect a little more perfect and is essential to accurate Western blot quantification. Your chosen normalization strategy and choice of reagents work together to ensure you are getting high-quality data for reliable normalization.
“Normalization: Improving the Imperfect” sets you up to get the most out of normalization by filling you in on:
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